Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)【科瑞奥博】

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Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)

货号： 9391S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	9391S
反应种属	Human/Mouse/Rat/All
来源宿主	Mouse
应用	W/IHC-P/E-P
目标/特异性	Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. It reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. The antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
使用方法	WB(1:5000) IHC-P (1:400) E-P (1:1000)

性能
供应商	CST
背景	The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.
存放说明	-20C

参考图片

Western blot analysis of extracts from Jurkat cells, untreated or nocadazole-treated (1 µg/ml for 12 hours prior to lysis), using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101). Proteins were separated by 2D electrophoresis prior to blotting.

对Jurkat细胞，未处理或1ug/ml nocadazole裂解前处理12小时，使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行Western blot分析。蛋白质在印记前在二维电泳分离。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phospho-threonine-proline motifs, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人乳腺癌使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行免疫组化分析，显示包含磷酸化苏氨酸-脯氨酸模体的蛋白染色。

Western blot analysis of extracts from COS cells, untreated or serum and okadaic acid-treated, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) (left). Right panel shows total protein staining.

对COS细胞，未处理或血清和冈田酸处理，使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)（左）进行Western blot分析。右侧显示全部蛋白染色。

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) ELISAs: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* denotes phosphorylated threonine.)

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) ELISA分析：磷酸化和非磷酸化的信噪比。（T*代表磷酸化的苏氨酸）

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人结肠癌使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Threonine-Prolin Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人乳腺癌对照（左）和λ磷酸酶处理（右），使用Phospho-Threonine-Prolin Mouse mAb (P-Thr-Pro-101)进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人膀胱变形上皮细胞癌使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行免疫组化分析。