| 货号 | 3004S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb detects endogenous levels of proteins containing phospho-threonine followed by proline and glutamine residues. This antibody also recognizes peptides and proteins containing the phospho-Thr-Pro motif. |
| 使用方法 | WB(1:1000) IP (1:100) E-P (1:1000) |
| 供应商 | CST |
| 背景 | The phospho-Thr-Pro-Glu motif is a subgroup of the phospho-Thr-Pro motif. The Thr-Pro motif is phosphorylated by proline-directed protein kinases, such as MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in many cellular processes, such as cell proliferation and differentiation (1-6). This phospho-Thr-Pro-Glu antibody provides a useful tool to study a subset of substrates of proline-directed kinases. |
| 存放说明 | -20C |
Western blot analysis of extracts from HeLa cells, untreated (-) or nocodazole-treated (100 ng/ml, 16 hr) (+), using Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb. 使用Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb对来自未处理（-）或100ng/ml Nocodazole处理16小时（+）的Hela细胞抽提液进行Western Blot分析。 | |
Immunoprecipitation of extracts from HeLa cells, untreated (-) or nocodazole-treated (100 ng/ml, 16 hr) (+), using Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb (lanes 3 and 4). Western blot analysis was performed using the same antibody. 10% input is shown in lanes 1 and 2. 使用Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb（列3和列4）对来自未处理（-）或100ng/ml Nocodazole处理16小时（+）的Hela细胞抽提液进行免疫共沉淀分析。Western Blot分析用相同抗体进行。10%输入显示在列1和2。 |
