| 货号 | 13072S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb recognizes endogenous levels of topoisomerase IIα protein only when phosphorylated at Ser1469. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 存放说明 | -20C |
| 计算分子量 | 190 |
Western blot analysis of extracts from 293T cells, untreated (-) or λ-phosphatase treated (+), and XP2Y0 cells, using Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb (top), Topoisomerase IIα (D10G9) XP® Rabbit mAb #12286 (middle) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (bottom).Western blot方法检测未经处理（-）和经λ-phosphatase处理过的293T和XP2Y0细胞提取物，使用的抗体为Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb (上图) ，Topoisomerase IIα (D10G9) XP® Rabbit mAb #12286（中图）或α-Actinin (D6F6) XP®Rabbit mAb #6487 (下图)。 | |
Confocal immunofluorescent analysis of 293T cells, untreated (left) or phosphatase-treated (center), and XP2YO cells (right) using Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). |
