Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb
货号：
12712S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 12712S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Thrombopoietin receptor (TPOR, c-Mpl) is a hematopoietic receptor that binds the growth factor, thrombopoietin (TPO), responsible for regulation of platelet production (1-3). Expression of TPOR by megakaryocytes is required for megakaryocyte growth and development (4). TPOR is also expressed by hematopoietic stem cells and is required for stem cell maintenance and expansion (5). Studies show that mice lacking either TPOR or TPO have severely reduced numbers of platelets and megakaryocytes as well as decreased numbers of other hematopoietic lineages (4,5). Binding of TPO to TPOR induces receptor dimerization that leads to phosphorylation and activation of the tyrosine kinase Jak2. Activated Jak2 associates with the cytoplasmic domain of TPOR (6,7) and phosphorylates TPOR at Tyr626 and Tyr631 (8). These phosphorylated tyrosine residues provide docking sites for downstream signaling molecules including Stat3, Stat5, Shc, and SHIP (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 85-90 |
参考图片Western blot analysis of extracts from serum-starved M-07e cells, untreated (-) or treated with recombinant human thrombopoietin (hTPO; 100 ng/mL, 10 min; +), using Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析缺乏血清的M-07e细胞提取物，未处理(-)或用重组人血小板生成素(hTPO; 100 ng/mL, 10 分钟; +)处理，使用的抗体是：Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb（上图）、β-Actin (D6A8) Rabbit mAb #8457（下图）。 |
Western blot analysis of extracts from serum-starved BaF3 cells or serum-starved BaF3 cells stably expressing mouse thrombopoietin receptor (mTPOR), untreated (-) or treated with recombinant mouse thrombopoietin (mTPO; 100 ng/mL, 10 min; +), using Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析缺乏血清的BaF3细胞或能稳定表达小鼠血小板生成素受体（mTPOR）的缺乏血清的BaF3细胞的提取物，未处理(-)或用重组小鼠血小板生成素(mTPO; 100 ng/mL, 10 分钟; +)处理，使用的抗体是：Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb（上图）、β-Actin (D6A8) Rabbit mAb #8457（下图）。 |
