| 货号 | 13041S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb recognizes endogenous levels of tyrosine hydroxylase protein only when phosphorylated at Ser31. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1600) |
| 供应商 | CST |
| 背景 | Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 55-60 |
Western blot analysis of extracts from PC-12 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb (upper) and Tyrosine Hydroxylase Antibody #2792 (lower). Western blot分析未处理(-)或经过λ 磷酸酶处理的PC-12细胞提取物，使用的抗体是Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗 (上) 和Tyrosine Hydroxylase Antibody 兔多抗 #2792 (下)。 | |
Western blot analysis of extracts from PC-12 cells, untreated (-) or treated with Roscovitine #9885 (20 μM, 16 hr; +), using Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb (upper) and Tyrosine Hydroxylase Antibody #2792 (lower). Western blot分析未处理(-)或经过Roscovitine #9885 (20 μM, 16 hr; +)处理的PC-12细胞提取物，使用的抗体是Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗(上) 和Tyrosine Hydroxylase Antibody 兔多抗#2792 (下)。 | |
Immunoprecipitation of phospho-tyrosine hydroxylase (Ser31) from mouse brain cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb. 对小鼠大脑细胞提取物的phospho-tyrosine hydroxylase (Ser31) 进行免疫沉淀，使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗(lane 3)。Lane1是10%上样量。使用 Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain, control (left) or λ phosphatase-treated (right), using Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb. 免疫组化分析对照(左)或λ 磷酸酶处理(+)的石蜡包埋的小鼠大脑切片，使用的抗体是Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded PC-12 cell pellets, control (left) or roscovitine and U0126-treated (right), using Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb. 免疫组化分析对照(左)或roscovitine和U0126处理(+)的石蜡包埋的PC-12细胞，使用的抗体是Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb. 免疫组化分析石蜡包埋的小鼠大脑切片，使用的抗体是Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb 兔单抗。 |
