| 货号 | 5869S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb detects endogenous levels of ULK1 only when phosphorylated at Ser555. Bands of unknown origin are detected between 90 and 100 kDa. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Two related serine/threonine kinases, UNC-51-like kinase -1 and -2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGap, and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16). |
| 存放说明 | -20C |
| 计算分子量 | 140-150 |
Western blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 μM, 30 minutes), and C2C12 cells, untreated or treated with hydrogen peroxide (10 mM, 5 minutes), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb. Western blot 分析MCF7细胞的细胞提取物，未处理或 oligomycin #9996 处理(0.5 μM, 30 分钟)；C2C12细胞的细胞提取物，未处理或过氧化氢处理(10 mM, 5 分钟)，使用抗体是 Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 µM, 30 minutes), using Phosho-ULK1 (Ser555) (D1H4) Rabbit mAb (left). Phoshpo-specificty is demonstrated by pre-incubating the antibody with phosphorylated (middle) or non-phoshporylated peptides (right) against a region surrounding Ser555 of ULK1. Western blot 分析MCF7细胞的细胞提取物，未处理或oligomycin #9996 (0.5 µM, 30 分钟)处理。使用抗体是Phosho-ULK1 (Ser555) (D1H4) Rabbit mAb 兔单抗(左图)。通过预孵育磷酸化的抗体(中图)或对应ULK1的Ser555周围残基的非磷酸化多肽(右图)验证磷酸化的特异性。 | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (100 μM, 2 hr; +), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower). |
