| 货号 | 14205S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Phosphorylation of ULK1 at Ser638 and Ser757 is mediated by mTOR, which is a regulator of cell growth and an inhibitor of autophagy that disrupts the interaction between ULK1 and AMPK (17,18). Conversely, AMPK is activated during low nutrient conditions and directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777 (17-19). |
| 存放说明 | -20C |
| 计算分子量 | 140-150 |
Western blot analysis of A172 cell extracts, untreated (-) or λ phosphatase and calf intestinal phosphatase (CIP)-treated (+), using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower). | |
Western blot analysis of various cell lines using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb. | |
Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (250 nM; 5 hr; +), or INK128-treated (250 nM; 5 hr; +) using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower). |
