| 货号 | 84519S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from A-431 and 3T3 cells, untreated (-) or Forskolin #3828-treated (10 μM, 15 min; +), using Phospho-VASP (Ser157) (D1C8O) Rabbit mAb (upper) or VASP (9A2) Rabbit mAb #3132 (lower). | |
Immunoprecipitation of Phospho-VASP (Ser157) from A-431 cell extracts treated with Forskolin #3828 (10 μM, 15 min). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-VASP (Ser157) (D1C8O) Rabbit mAb. Western blot analysis was performed using Phospho-VASP (Ser157) (D1C8O) Rabbit mAb. A light chain-specific secondary antibody was used. |
