Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
货号：
2476S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2476S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb detects transfected levels of VEGFR-2 only when phosphorylated at tyrosine 951. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
参考图片Western blot analysis of extracts from CKR/PAE cells expressing chimeric receptors containing human CSF-1 receptor extracellular binding domain/mouse VEGF receptor 2 intracellular domian (Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992), using Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb.用Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb对含有人CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体的CKR/PAE细胞的提取物进行免疫印迹检测(Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992)。 |
