| 货号 | 4910S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8).所有真核细胞进入有丝分裂都经由cdc2激酶激活的调节。激活cdc2进入有丝分裂进程的关键的调节步骤似乎是15位和14位酪氨酸的去磷酸化（1,2）。15位和14位酪氨酸的磷酸化和cdc2抑制是通过Wee1和MYT1蛋白激酶来执行的，而15位酪氨酸的去磷酸化和cdc2活化是通过cdc25磷酸酶来执行（1,3,4）。有丝分裂中Myt1的过度磷酸化表明G2/ M转换期一个或多个激酶的激活负向调节Myt1活性。已经证实的磷酸化Myt1的激酶包括cdc2，p90RSK，Akt和Plk1 (5-8)。 |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of extracts from A431 and H441 cells, untreated or EGF-treated, using Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb (upper) or Wee1 Antibody #4936 (lower).Western blot检测非处理和EGF处理的A431和H441细胞提取物，使用的抗体为Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb (上图) 或Wee1 Antibody #4936 (下图)。 | |
Immunoprecipitation of phospho-wee1 from A431 cells, untreated or EGF-treated, using Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb followed by Western blot using the same antibody.从A431细胞中免疫沉淀磷酸化wee1蛋白，分为非处理组和EGF处理组，使用的抗体为Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb ，随后western blot使用同一抗体。 |
