| 货号 | 8467S |
| 反应种属 | Human/Mouse/Rat/Monkey/Dog |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142 and Ser143. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | The zyxin family of proteins includes LIMD1, ajuba, trip6, and zyxin, each of which contains three LIM domains at the carboxy terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2) and in the regulation of adhesion and migration, possibly through the recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Zyxin may be phosphorylated by Akt at Ser142 (5). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from A-431 and U-2 OS cells, untreated (-) or treated (+) with λ and calf intestinal phosphatase (λ/CIP), using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb (upper) or Zyxin Antibody #3553 (lower).未处理（-）或经过λ和牛小肠碱性磷酸酶（λ/CIP）联合处理的A-431细胞提取物，使用Phospho-Zyxin (Ser142/143) (D1E8)兔单抗（上）或Zyxin抗体#3553（下）进行western blot分析。 | |
Immunoprecipitation of extracts from A-431 cells using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 10% input.使用Phospho-Zyxin (Ser142/143) (D1E8)兔单抗对A-431细胞提取物进行免疫共沉淀。western blot使用同样的抗体。第1道使用10% input。 |
