Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb【科瑞奥博】

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Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb

货号： 2416S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	2416S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Mouse
应用	W/IP
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
背景	Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (or β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
存放说明	-20C
计算分子量	50

参考图片

Western blot analysis of extracts from HEK293 cells alone (-), expressing β-arrestin 1 (wt) or β-arrestin (Ser412Ala) mutant (Ala412), using Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb. Lanes 1 and 3 show endogenous levels of phosphorylated β-arrestin 1.western blot方法检测细胞提取物：只选用能表达β-arrestin 1 (wt) 和 β-arrestin (Ser412Ala)突变体(Ala412) 的HEK293细胞 (-)，使用的抗体是Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb。1、3条带表示内源性磷酸化的 β-arrestin 1蛋白水平。

Immunoprecipitation of β-arrestin 1 from HEK293 cells using a polyclonal antibody to phospho-β-arrestin 1 (Ser412), followed by alkaline phosphaphatase (CIP) treatment. β-arrestin 1 was detected by Phospho-beta-Arrestin 1 (Ser412) (6-24) Mouse mAb. CIP treatment abolished the β-arrestin 1 signal, indicating that the monoclonal antibody is phospho-specific.免疫沉淀方法检测细胞提取物：HEK293 细胞中的β-arrestin 1 蛋白，使用的抗体是phospho-β-arrestin 1（Ser412)单克隆抗体，接着用碱性磷酸酶（CIP）处理。用Phospho-beta-Arrestin 1 (Ser412) (6-24) Mouse mAb检测β-arrestin 1蛋白。CIP处理后会使β-arrestin 1蛋白信号消除，表明该单抗是一种特异性磷酸根抗体。