| 货号 | 29021S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Phospho-PLCbeta3 (Ser537) (D8K2R) Rabbit mAb recognizes endogenous levels of total PLCbeta3 only when phosphorylated at Ser537. The antibody cross-reacts with a murine protein of ~75 kDa whose identity was not determined. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. 2 . Smrcka, A.V. et al. (1991) Science 251, 804-7. 3 . Taylor, S.J. et al. (1991) Nature 350, 516-8. 4 . Yue, C. et al. (1998) J Biol Chem 273, 18023-7. 5 . Yue, C. et al. (2000) J Biol Chem 275, 30220-5. 6 . Margolis, B. et al. (1989) Cell 57, 1101-7. 7 . Kim, H.K. et al. (1991) Cell 65, 435-41. 8 . Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7. |
Western blot analysis of extracts from HeLa cells untreated (-) or treated with anisomycin (20 μg/ml for 15 min, +), or λ phosphatase-treated (+), using Phospho-PLCbeta3 (Ser537) (D8K2R) Rabbit mAb (upper), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). |
