Tri-Methyl Lysine Motif [tme-K] (D1L1X) Rabbit mAb
货号：
14680S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 14680S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Methylation of lysine residues is a common regulatory posttranslational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). Two PKMT groups are recognized based on structure and catalytic mechanism: class I methyltransferases or seven β strand enzymes, and SET domain-containing class V methyltransferases. Both use the methyl donor S-adenosyl-L-methionine to methylate histone and non-histone proteins. Class I methyltransferases methylate amino acids, DNA, and RNA (1,2). Six methyl-lysine-interacting protein families are distinguished based on binding domains: MBT, PHD finger, Tudor, PWWP, WD40 repeat, and chromodomains. Many of these display differential binding preferences based on lysine methylation state (3). KDM1 subfamily lysine demethylases catalyze demethylation of mono- and di-methyl lysines, while 2-oxoglutarate-dependent JmjC (KDM2-7) subfamily enzymes also modify tri-methyl lysine residues (4). |
| 存放说明 | -20C |
参考图片The specificity of Tri-Methyl Lysine Motif [tme-K] (D1L1X) Rabbit mAb was determined using peptide ELISA. The figure demonstrates that the antibody is specific for tri-methyl lysine and does not cross-react with mono- or di-methyl lysine or arginine. |
Western blot analysis of HeLa cells, untreated (-) or treated with adenosine-2,3-dialdehyde (AdOx, 100 μM, 24hr; +), using Tri-Methyl Lysine Motif [tme-K] (D1L1X) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
