| 货号 | 13439S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) mouse mAb has been used to assess PCNA levels in hundreds of scientific studies. 增殖细胞核抗原（PCNA）是DNA滑动钳蛋白家族的一员，协助DNA复制（1）。晶体结构数据提示PCNA形成的同源三聚体环可以环绕包围DNA双螺旋（2）。很多涉及到DNA复制，修复和细胞周期调控的蛋白都是与PCNA结合而不是直接地和DNA结合，因此可以促进DNA的快速处理（reviewed in 3）。PCNA蛋白表达是一个广泛认可的增殖信号，PCNA（PC10）mouse mAb已经被上百种研究用于衡量PCNA的表达量。 |
| 存放说明 | -20C |
| 计算分子量 | 44 |
Immunoprecipitation of Ubiquityl-PCNA (Lys164) from UV-treated (100 mJ, 1 hr recovery) HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb.来自UV-处理(100 mJ, 1 hr 恢复)HeLa细胞的Ubiquityl-PCNA (Lys164)，使用Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) 或Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (lane 3)进行免疫沉淀实验。Lane1是10%上样量。使用Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (100 mJ, 1 hr recovery; +), using Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (upper) or PCNA (PC10) Mouse mAb #2586 (lower).未处理(-) 或 UV-处理(100 mJ, 1 hr 恢复; +),的HeLa细胞提取物，使用Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (上)或 PCNA (PC10) Mouse mAb #2586 (下)进行western blot分析。 |
