| 货号 | 4376S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Mink/D.melanogaster/Zebrafish/Pig/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
Western blot analysis of active p42 MAP Kinase, active p38 MAPK and active JNK2 proteins, as well as extracts from PDGF-treated NIH/3T3 cells and EGF-treated A431 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb.western blot方法检测：活化的p42 MAP Kinase、p38 MAPK、 JNK2蛋白，以及PDGF处理的NIH/3T3细胞和EGF处理的A431细胞，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织。使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb。 | |
Immunohistochemical analysis of MDA-MB-453 cells, untreated, EGF-treated or EGF/Tarceva-treated, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb.免疫组织化学方法检测MDA-MB-453细胞，左侧是未处理的样品，右侧是EGF/Tarceva处理后的样品。使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated or λ phosphatase-treated (right), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，左侧样品未经处理，右侧样品经λ磷酸酶处理。使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human prostate tumor, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb (left) or the same antibody pre-incubated with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide #1150 (right).免疫组织化学方法检测石蜡包埋的人前列腺癌组织。左侧使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb，右侧表示将相同抗体提前与Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide #1150孵育。 |
