| 货号 | 16564S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IHC,IF |
| 目标/特异性 | MUC1-C (D5K9I) XP® Rabbit mAb recognizes endogenous levels of total MUC1-C protein. This antibody does not detect MUC1-N or full-length (uncleaved) MUC1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunohistochemistry (Paraffin) (1:200) Immunofluorescence (Immunocytochemistry) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6) and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8). MUC1-C is the carboxy-terminal transmembrane subunit of MUC1 resulting from proteolytic cleavage of the full length protein. MUC1-N is the amino-terminal subunit, which can be tethered to MUC1-C, or released from the plasma membrane. MUC1-C interacts with receptor tyrosine kinases, β-catenin and other signaling proteins, and is thought to induce activation of MAPK, Akt and Wnt pathways. Due to its signaling functions and expression in human cancer, MUC1-C is a potential therapeutic target (reviewed in 9). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
| 参考文献 | 1 . Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231. 2 . Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64. 3 . Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24. 4 . Li, Y. et al. (2001) J Biol Chem 276, 6061-4. 5 . Ren, J. et al. (2002) J Biol Chem 277, 17616-22. 6 . Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47. 7 . Raina, D. et al. (2004) J Biol Chem 279, 20607-12. 8 . Wei, X. et al. (2005) Cancer Cell 7, 167-78. 9 . Kufe, D.W. (2013) Oncogene 32, 1073-81. |
Immunohistochemical analysis of paraffin-embedded ZR-75-1 (left, positive) and HCT 116 (right, negative) cell pellets using MUC1-C (D5K9I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using MUC1-C (D5K9I) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using MUC1-C (D5K9I) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of T-47D (left, positive) and SK-MEL-28 (right, negative) cells using MUC1-C (D5K91) XP® Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MUC1-C (D5K9I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MUC1-C (D5K9I) XP® Rabbit mAb. |
