| 货号 | 70109S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M Mk |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P F |
| 目标/特异性 | Jagged1 (D4Y1R) XP® Rabbit mAb recognizes endogenous levels of total Jagged1 protein. Based on sequence analyses, this antibody is not predicted to detect Jagged2 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:200) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 180 |
| 参考文献 | 1 . Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868. 2 . Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328. 3 . Chiba, S. (2006) Stem Cells 24, 2437-2447. 4 . Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610. 5 . Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643. 6 . LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437. |
Western blot analysis of extracts from various cell lines using Jagged1 (D4Y1R) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Molt4 and Jurkat cells do not expression Jagged1 protein, confirming specificity of the antibody for Jagged1. | |
Flow cytometric analysis of Jurkat cells (blue) and MKN45 cells (green) using Jagged1 (D4Y1R) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using Jagged1 (D4Y1R) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Jagged1 (D4Y1R) XP(R) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunoprecipitation of Jagged1 protein from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) XP® IgG Isotype Control #3900, and lane 3 is Jagged1 (D4Y1R) XP® Rabbit mAb. Western blot analysis was performed using Jagged1 (D4Y1R) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HepG2 (positive, left) and Jurkat (negative, right) cell pellets using Jagged1 (D4Y1R) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jagged1 (D4Y1R) XP® Rabbit mAb. |
