| 货号 | 93668S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W IHC-P IF-F IF-IC |
| 目标/特异性 | Arginase-1 (D4E3M™) XP® Rabbit mAb recognizes endogenous levels of total arginase-1 protein. This antibody does not cross-react with arginase-2. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | L-arginine plays a critical role in regulating the immune system (1-3). In inflammation, cancer and certain other pathological conditions, myeloid cell differentiation is inhibited leading to a heterogeneous population of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs) (4). MDSCs are recruited to sites of cancer-associated inflammation and express high levels of arginase-1 (4). Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine and urea (5). Thus MDSCs increase the catabolism of L-arginine resulting in L-arginine depletion in the inflammatory microenvironment of cancer (4, 6). The reduced availability of L-arginine suppresses T-cell proliferation and function and thus contributes to tumor progression (4, 6). Arginase-1 is of great interest to researchers looking for a therapeutic target to inhibit the function of MDSCs in the context of cancer immunotherapy (7). In addition, research studies have demonstrated that Arginase-1 distinguishes primary hepatocellular carcinoma (HCC) from metastatic tumors in the liver, indicating its value as a potential biomarker in the diagnosis of HCC (8, 9). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Albina, J.E. et al. (1989) J Exp Med 169, 1021-9. 2 . Mills, C.D. (2001) Crit Rev Immunol 21, 399-425. 3 . Rodriguez, P.C. et al. (2004) Cancer Res 64, 5839-49. 4 . Gabrilovich, D.I. and Nagaraj, S. (2009) Nat Rev Immunol 9, 162-74. 5 . Wu, G. and Morris, S.M. (1998) Biochem J 336 ( Pt 1), 1-17. 6 . Raber, P. et al. (2012) Immunol Invest 41, 614-34. 7 . Wesolowski, R. et al. (2013) J Immunother Cancer 1, 10. 8 . Sang, W. et al. (2015) Tumour Biol 36, 3881-6. 9 . Geramizadeh, B. and Seirfar, N. (2015) Hepat Mon 15, e30336. |
Western blot analysis of extracts from mouse liver and mouse small intestine using Arginase-1 (D4E3M™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded mouse liver using Arginase-1 (D4E3M™) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) using Arginase-1 (D4E3M™) XP® Rabbit mAb (green). BMDMs were differentiated with M-CSF (20 ng/ml, 7 days) and activated with either IL-4/cAMP (20 ng/ml, 0.5 mM, 24 hours; left) or LPS/IFNγ (50 ng/ml, 20 ng/ml, 24 hours; right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. | |
Confocal immunofluorescent analysis of mouse liver (positive; left) or small intestine (negative; right) using Arginase-1 (D4E3M™) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from mouse and rat liver using Arginase-1 (D4E3M™) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded normal human liver using Arginase-1 (D4E3M™) XP® Rabbit mAb. |
