| 货号 | 30853S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | WB,IP,IF |
| 目标/特异性 | Emerin (D3B9G) XP® Rabbit mAb recognizes endogenous levels of total emerin protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunofluorescence (Immunocytochemistry) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that emerin is required for HIV-1 infectivity (9). Mutations in the gene encoding emerin (EMD) are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10). |
| 存放说明 | -20C |
| 计算分子量 | 30, 35 |
| 参考文献 | 1 . Nagano, A. et al. (1996) Nat. Genet. 12, 254-259. 2 . Manilal, S. et al. (1998) Biochem. Biophys. Res. Commun. 249, 643-647. 3 . Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 709-714. 4 . Lee, K.K. et al. (2001) J. Cell Sci. 114, 4567-4573. 5 . Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17, 1154-1163. 6 . Holaska, J.M. et al. (2003) J. Biol. Chem. 278, 6969-6975. 7 . Haraguchi, T. et al. (2004) Eur. J. Biochem. 271, 1035-1045. 8 . Markiewicz, E. et al. (2006) EMBO J. 25, 3275-3285. 9 . Jacque, J.M. and Stevenson, M. (2006) Nature 441, 641-645. 10 . Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 676-680. |
Western blot analysis of extracts from various cell lines using Emerin (D3B9G) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of emerin from HeLa cell extracts using Emerin (D3B9G) XP® Rabbit mAb. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Emerin (D3B9G) XP® Rabbit mAb. Western blot analysis was performed using Emerin (D3B9G) XP® Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the western blot secondary antibody. | |
Confocal immunofluorescent analysis of HeLa cells (left, positive) or THP-1 cells (right, negative) using Emerin (D3B9G) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
