| 货号 | 86781S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IHC |
| 目标/特异性 | CD133 (D4W4N) XP® Rabbit mAb recognizes endogenous levels of total CD133 protein. The antibody is not sensitive to the glycosylation status of CD133. |
| 使用方法 | Western Blotting (1:1000) IHC-Leica® Bond™ (1:700) Immunohistochemistry (Paraffin) (1:700) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8). |
| 存放说明 | -20C |
| 计算分子量 | 133 |
| 参考文献 | 1 . Yin, A.H. et al. (1997) Blood 90, 5002-12. 2 . Miraglia, S. et al. (1997) Blood 90, 5013-21. 3 . Handgretinger, R. et al. (2003) Ann N Y Acad Sci 996, 141-51. 4 . Monzani, E. et al. (2007) Eur J Cancer 43, 935-46. 5 . OBrien, C.A. et al. (2007) Nature 445, 106-10. 6 . Ricci-Vitiani, L. et al. (2007) Nature 445, 111-5. 7 . Singh, S.K. et al. (2004) Nature 432, 396-401. 8 . Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34. |
Western blot analysis of extracts from various cell lines using CD133 (D4W4N) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from WERI-Rb-1 cells, untreated (-) or treated with PNGase F (+) using CD133 (D4W4N) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD133 (D4W4N) XP® Rabbit mAb performed on the Leica® BOND™ Rx. | |
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using CD133 (D4W4N) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD133 (D4W4N) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human kidney using CD133 (D4W4N) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human pancreas using CD133 (D4W4N) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Caco-2 (left) and HeLa (right) cell pellets using CD133 (D4W4N) XP® Rabbit mAb. |
