| 货号 | 83535S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP,IHC,IF |
| 目标/特异性 | WT1 (D8I7F) XP® Rabbit mAb recognizes endogenous levels of total WT1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunohistochemistry (Paraffin) (1:100) Immunofluorescence (Immunocytochemistry) (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Wilms Tumor 1 (WT1) is a transcription factor named from Wilms Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the WT1 gene (1). It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of expression of WT1 result in a variety of syndromes affecting the genitourinal system and other tissues (2-5). WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post translation modifications of the protein (9). |
| 存放说明 | -20C |
| 计算分子量 | 51-60 |
| 参考文献 | 1 . Royer-Pokora, B. et al. (2004) Am J Med Genet A 127A, 249-57. 2 . Kohsaka, T. et al. (1999) Hum Mutat 14, 466-70. 3 . Little, M. et al. (2000) Hum Mutat 15, 389. 4 . Takata, A. et al. (2000) J Med Genet 37, 698-701. 5 . Suri, M. et al. (2007) Am J Med Genet A 143A, 2312-20. 6 . Toska, E. and Roberts, S.G. (2014) Biochem J 461, 15-32. 7 . Yang, L. et al. (2007) Leukemia 21, 868-76. 8 . Weiss, T.C. and Romaniuk, P.J. (2009) Biochemistry 48, 148-55. 9 . Haber, D.A. et al. (1991) Proc Natl Acad Sci U S A 88, 9618-22. |
Western blot analysis of extracts from K-562 and OVCAR3 cells using WT1 (D8I7F) XP® Rabbit mAb. | |
Immunoprecipitation of WT1 from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is WT1 (D8I7F) XP® Rabbit mAb. Western blot analysis was performed using WT1 (D8I7F) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using WT1 (D8I7F) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using WT1 (D8I7F) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using WT1 (D8I7F) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded OVCAR3 cell pellet (left, positive) or DU 145 cell pellet (right, negative) using WT1 (D8I7F) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of OVCAR3 cells (left, positive) and DU 145 cells (right, negative) using WT1 (D8I7F) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). |
