| 货号 | 39141S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-F |
| 目标/特异性 | Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse Olfm4 protein. |
| 使用方法 | W IP IHC-P IF-F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Olfactomedin-4 (OLFM4, hGC-1) is a member of the Olfactomedin family, a small group of extracellular proteins defined by the presence of a conserved "Olfactomedin domain" that is thought to facilitate protein-protein interactions (1). OLFM4 is a secreted glycoprotein, which forms disulfide bond-mediated oligomers, and is thought to mediate cell adhesion through its interactions with extracellular matrix proteins such as lectins (2). Human OLFM4was first cloned from myeloid cells (3) and is expressed in a distinct subset of neutrophils, though the functional significance of this differential expression pattern remains unclear (4). Among normal tissues, the expression of OLFM4 protein is most abundant in intestinal crypts (5), where it has garnered attention as a possible marker of intestinal stem cells (6). Notably, OLFM4 expression is markedly increased in several tumor types, including colorectal, gastric, pancreas, lung, and breast (reviewed in [1]). Furthermore, research studies show that the expression levels of OLFM4 vary in relation to the severity and/or differentiation status of multiple tumor types (1, 6-8), leading to the suggestion that OLFM4 may have utility as a prognostic marker in some cancer patients (9). |
| 存放说明 | -20C |
| 计算分子量 | 85-90, 70 |
| 参考文献 | 1 . Yu, L. et al. (2011) Neoplasma 58, 9-13. 2 . Liu, W. et al. (2006) Exp Cell Res 312, 1785-97. 3 . Zhang, J. et al. (2002) Gene 283, 83-93. 4 . Welin, A. et al. (2013) PLoS One 8, e69575. 5 . van der Flier, L.G. et al. (2009) Gastroenterology 137, 15-7. 6 . Liu, W. et al. (2007) Histopathology 51, 157-65. 7 . Liu, W. et al. (2008) Clin Cancer Res 14, 1041-9. 8 . Besson, D. et al. (2011) Mol Cell Proteomics 10, M111.009712. 9 . Seko, N. et al. (2010) Exp Ther Med 1, 73-8. |
Western blot analysis of extracts from mouse small intestine (positive), mouse spleen (positive), and mouse colon (negative) using Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of mouse small intestine using Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded normal mouse small intestine (left) and colon (right) using Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific). | |
Immunohistochemical analysis of paraffin-embedded wild-type (left) and Olfm4 knockout (right) mouse spleen using Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific). (Tissue courtesy of Dr. Wenli Lui and Dr. Griffin P. Rodgers, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD). | |
Immunohistochemical analysis of paraffin-embedded APC (Min/+) mouse intestinal adenoma using Olfm4 (D6Y5A) XP® Rabbit mAb (Mouse Specific). |
