| 货号 | 77274S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , F , IF-IC |
| 目标/特异性 | CD74 (D5N3I) XP® Rabbit mAb recognizes endogenous levels of total CD74 protein. |
| 使用方法 | W IHC-P IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD74, which is also known as the MHC Class II-associated invariant chain (Ii), is a type II transmembrane glycoprotein that plays a critical role in the antigen presentation process as a chaperone of MHC Class II proteins. It is expressed at high levels on B cells and to a lesser extent on numerous antigen presenting cell (APC) types including dendritic cells, Langerhans cells, monocytes, and macrophages as well as non-traditional APCs such as epithelial cells (1,2). CD74 was initially identified for its ability to regulate the folding and intracellular trafficking of newly synthesized MHC Class II molecules. Following expression, CD74 self-assembles as a trimer that serves as a scaffold for the assembly of MHC Class II molecules. Through this interaction, CD74 blocks the peptide binding cleft of MHC Class II molecules and prevents their premature association with endogenous polypeptides (3). Binding to CD74 also facilitates the translocation of MHC Class II molecules from the endoplasmic reticulum to the endocytic compartments during antigen presentation (4). In addition to its role as an MHC Class II chaperone, CD74 is also the receptor for macrophage migration-inhibitory factor (MIF). Binding to CD74 and its co-receptor, CD44, has been shown to induce the activation of the NFkB and ERK pathways to promote cell proliferation and survival signals (5,6). Recent studies have identified CXCR2 and CXCR4 as co-receptors for CD74 where MIF binding to CD74 complexes contributes to MIF-mediated monocyte chemotaxis and the induction of Akt signaling, respectively (7,8). Increased CD74 surface expression has been reported under inflammatory conditions and in certain types of cancer cells implying a potential role in tumorigenesis (9). |
| 存放说明 | -20C |
| 计算分子量 | 20-25, 30-40 |
| 参考文献 | 1 . Barrera, C.A. et al. (2005) J Histochem Cytochem 53, 1481-9. 2 . Lotteau, V. et al. (1990) Nature 348, 600-5. 3 . Stockinger, B. et al. (1989) Cell 56, 683-9. 4 . Bakke, O. and Dobberstein, B. (1990) Cell 63, 707-16. 5 . Shi, X. et al. (2006) Immunity 25, 595-606. 6 . Starlets, D. et al. (2006) Blood 107, 4807-16. 7 . Bernhagen, J. et al. (2007) Nat Med 13, 587-96. 8 . Schwartz, V. et al. (2009) FEBS Lett 583, 2749-57. 9 . Borghese, F. and Clanchy, F.I. (2011) Expert Opin Ther Targets 15, 237-51. |
Confocal immunofluorescent analysis of Raji (left) and Jurkat (right) cells using CD74 (D5N3I) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human endometrioid ovarian carcinoma using CD74 (D5N3I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human spleen using CD74 (D5N3I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD74 (D5N3I) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using CD74 (D5N3I) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD74 (CD74-Myc/DDK; +), using CD74 (D5N3I) Rabbit XP®mAb. | |
Flow Cytometric analysis of human blood mononuclear cells using CD74 (D5N3I) Rabbit mAb (right) co-stained with anti-human CD19-APC compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (left). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Aanalysis was performed on gated lymphocyte cells. | |
Flow Cytometric analysis of Raji cells (green), HT29 cells (red) and Jurkat cells (blue) using CD74 (D5N3I) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
