| 货号 | 83700S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC , ChIP |
| 目标/特异性 | NKX3.1 (D2Y1A) XP® Rabbit mAb recognizes endogenous levels of total NKX3.1 protein. |
| 使用方法 | W IF-IC ChIP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | NKX3.1 is a homeobox transcription factor that in mammals plays a defining role in embryonic prostate morphogenesis. The expression of mammalian NKX3.1 is androgen-dependent, restricted primarily to developing and mature prostate epithelium, and is frequently reduced or lost in prostate cancer (1-3). The human NKX3.1 gene is located on chromsome 8p21.2, within a region that shows loss of heterozygosity (LOH) in >50% of prostate cancer cases (2). Allelic loss at the NKX3.1 locus is also common in high grade Prostate Intraepithelial Neoplasia (PIN), thought to be a putative precursor lesion to invasive prostate adenocarcinomas, suggesting that LOH at the NKX3.1 locus is a critical early step in prostate cancer development (4). Notably, the remaining NKX3.1 allele is intact in the majority of LOH cases, leading to the suggestion that NKX3.1 functions as a haploinsufficient tumor suppressor (4-6). Due to its highly restricted expression in prostate epithelial cells, NKX3.1 has been suggested as a diagnostic marker of prostate carcinoma (7), and may have additional utility as a biomarker of metastatic lesions originating in the prostate (8). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Bhatia-Gaur, R. et al. (1999) Genes Dev 13, 966-77. 2 . He, W.W. et al. (1997) Genomics 43, 69-77. 3 . Bowen, C. et al. (2000) Cancer Res 60, 6111-5. 4 . Magee, J.A. et al. (2003) Cancer Cell 3, 273-83. 5 . Voeller, H.J. et al. (1997) Cancer Res 57, 4455-9. 6 . Bethel, C.R. et al. (2006) Cancer Res 66, 10683-90. 7 . Epstein, J.I. et al. (2014) Am J Surg Pathol 38, e6-e19. 8 . Conner, J.R. and Hornick, J.L. (2015) Adv Anat Pathol 22, 149-67. |
Western blot analysis of extracts from human prostate and various cell lines using NKX3.1 (D2Y1A) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). DND-41 cell are negative for NKX3.1 expression, confirming specificity of the antibody. | |
Confocal immunofluorescent analysis of LNCaP (left; positive) and HeLa (right; negative) cells using NKX3.1 (D2Y1A) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 LNCaP cells and either 5 μl of NKX3.1 (D2Y1A) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CYP2B7P Pseudogene Promoter Primers #84846, human MALSU1 downstream primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
