| 货号 | 5601S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | βIG-H3 (D31B8) XP® Rabbit mAb detects endogenous levels of total βIG-H3 |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β-1 #8915 (100 ng/ml for 24 hours, right), using βIG-H3 (D31B8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对未处理（左）或hTGF-β-1 #8915 (100 ng/ml 24 小时, 右)处理的A549细胞进行激光共聚焦荧光分析，使用 βIG-H3 (D31B8) XP®兔单抗（绿色）进行实验。肌动蛋白丝使用DY-554鬼笔环肽标记。蓝色假色=DRAQ5® #4084 （DNA荧光染料）。 | |
Western blot analysis of extracts from A549 cells treated with hTGFβ-3 #8425 (100 ng/ml for 12 hours) and OVCAR8 cells using βIG-H3 (D31B8) XP® Rabbit mAb.使用hTGFβ-3 #8425（100 ng/ml 12小时）处理的A549细胞和OVCAR8细胞提取物进行western blot分析，使用βIG-H3 (D31B8) XP®兔单抗进行实验。 |
