| 货号 | 8243S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-P |
| 目标/特异性 | β-Amyloid (D54D2) XP® Rabbit mAb recognizes endogenous levels of total β-amyloid peptide (Aβ). The antibody detects several isoforms of Aβ, such as Aβ-37, Aβ-38, Aβ-39, Aβ-40, and Aβ-42. The antigen for this antibody is not 100% conserved in rat. However, the antibody detects mouse Aβ and the mouse and rat sequences surrounding the antigen are 100% identical. |
| 使用方法 | WB(1:1000) IP (1:50) IF-P (1:100) |
| 供应商 | CST |
| 背景 | Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimers disease (AD) (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 5 |
Confocal immunofluorescent analysis of paraffin-embedded human Alzheimers brain using β-Amyloid (D54D2) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析石蜡包埋的人阿尔茨海默病患者的大脑切片，使用的抗体为 β-Amyloid (D54D2) XP® Rabbit mAb兔单抗（绿色）和Tau (Tau46) Mouse mAb #4019鼠单抗（红色）。蓝色伪彩=DRAQ5® #4084 荧光DNA染料)。 | |
Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb. Western blot检测AD病人的Aβ-42肽段(1 ng) 和脑脊液(CSF) ，使用的抗体为β-Amyloid (D54D2) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb. Western blot 检测人Aβ-42，Aβ-40，Aβ-39，Aβ-38和Aβ-37肽段(5 ng)，使用的抗体为β-Amyloid (D54D2) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb. Western blot定量检测人Aβ-42 （左）和Aβ-40（右）肽段，使用的抗体为β-Amyloid (D54D2) XP® Rabbit mAb兔单抗。 |
