| 货号 | 5568S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/IF-IC |
| 目标/特异性 | β3-Tubulin (D71G9) XP® Rabbit mAb detects endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Confocal immunofluorescent analysis of mouse cerebellum using β3-Tubulin (D71G9) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析小鼠小脑，使用的抗体是β3-Tubulin(D71G9) XP® Rabbit mAb 兔单抗（绿色）和Tau (Tau46)Mouse mAb 鼠单抗#4019（红色）。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of HeLa and human cerebellum using β3-Tubulin (D71G9) XP® Rabbit mAb. Western blot分析HeLa和人小脑提取物，使用的抗体是β3-Tubulin(D71G9)XP® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of P19 cells that were differentiated with retinoic acid, using β3-Tubulin (D71G9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 #4084® (fluorescent DNA dye). |
