| 货号 | 3704S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | ZO-3 (D57G7) XP® Rabbit mAb detects endogenous levels of total ZO-3 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from MCF-7, HT-29 and HCT-15 cells using ZO-3 (D57G7) XP® Rabbit mAb.使用ZO-3 (D57G7) XP® 兔单抗对MCF-7, HT-29 和 HCT-15细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of MCF-7 cells using ZO-3 (D57G7) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用ZO-3 (D57G7) XP®兔单抗（绿色）对MCF-7细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084（DNA荧光染料）。 |
