| 货号 | 64953S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:200) F (1:100) |
| 供应商 | CST |
| 背景 | VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 45-65 |
Immunohistochemical analysis of paraffin-embedded human kidney using VISTA (D1L2G) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human tonsil using VISTA (D1L2G) XP® Rabbit mAb. | |
Western blot analysis of extracts from OVKATE, DU 145, and MCF7 cells using VISTA (D1L2G) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded OVKATE (left) and MCF7 (right) cell pellets using VISTA (D1L2G) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins B-Cell lymphoma using VISTA (D1L2G) XP®Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using VISTA (D1L2G) XP®Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using VISTA (D1L2G) XP®Rabbit mAb. | |
Flow cytometric analysis of human whole blood cells stained with VISTA (D1L2G) XP® Rabbit mAb and co-stained with CD19-PE and CD14-APC. VISTA is present on CD14+ monocytes (right; upper right quadrant) and shows a significant increase in intensity when compared to its isotype control (middle; upper left quadrant). CD19+ B cells are negative for VISTA (left; upper left quadrant). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
