| 货号 | 13901S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 使用方法 | WB(1:1000) IHC-P (1:150) F (1:400) |
| 供应商 | CST |
| 背景 | Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6). This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8). This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9). |
| 存放说明 | -20C |
| 计算分子量 | 124 |
Immunohistochemical analysis of paraffin-embedded Hs578T (left) and Jurkat (right) cell pellets using Vinculin (E1E9V) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using Vinculin (E1E9V) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Vinculin (E1E9V) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb, #6487 (lower). | |
Flow cytometric analysis of Jurkat cells (blue) and HeLa cells (green) using Vinculin (E1E9V) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma in situ using Vinculin (E1E9V) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Vinculin (E1E9V) XP® Rabbit mAb. |
