| 货号 | 5741S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Vimentin (D21H3) XP® Rabbit mAb detects endogenous levels of total vimentin protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentins dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7). |
| 存放说明 | -20C |
| 计算分子量 | 57 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vimentin (D21H3) XP® Rabbit mAb. 使用Vimentin (D21H3) XP® Rabbit mAb，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用Vimentin (D21H3) XP® Rabbit mAb，提前与control peptide (左图)或antigen-specific peptide (右图)孵育，免疫组化分析人源扁桃体组织石蜡切片。 | |
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb. 使用Vimentin (D21H3) XP® Rabbit mAb兔单抗，免疫印迹（Western Blot）分析不同细胞中Vimentin蛋白水平。 | |
Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)比较，使用Vimentin (D21H3) XP® Rabbit mAb (蓝色)，流式细胞仪分析HeLa细胞。 | |
Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb. |
