| 货号 | 91590S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IHC |
| 目标/特异性 | Aurora A (D3V7T) XP®Rabbit mAb recognizes endogenous levels of total Aurora A protein. |
| 使用方法 | Western Blotting (1:1000) Immunohistochemistry (Paraffin) (1:500) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C (1,2). Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression (3). Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation (4,5); research investigators have observed overexpression of these kinases in a variety of human cancers (2,4). Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines (6). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Warner, S.L. et al. (2003) Mol Cancer Ther 2, 589-95. 2 . Katayama, H. et al. (2003) Cancer Metastasis Rev 22, 451-64. 3 . Andrews, P.D. et al. (2003) Curr Opin Cell Biol 15, 672-83. 4 . Pascreau, G. et al. (2003) Prog Cell Cycle Res 5, 369-74. 5 . Crosio, C. et al. (2002) Mol Cell Biol 22, 874-85. 6 . Kimura, M. et al. (1999) J Biol Chem 274, 7334-40. |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins lymphoma using Aurora A (D3V7T) XP® Rabbit mAb. | |
Western blot analysis of extracts from HT-29 and HeLa cells, untreated (-) or treated with nocodazole (100 ng/mL, 16 hr; +), using Aurora A (D3V7T) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Aurora A (D3V7T) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Aurora A (D3V7T) XP® Rabbit mAb. |
