| 货号 | 90176S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,IP,IHC |
| 目标/特异性 | CD19 (D4V4B) XP® Rabbit mAb recognizes endogenous levels of total CD19 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) IHC-Leica® Bond™ (1:400) Immunohistochemistry (Paraffin) (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Tedder, T.F. et al. (1997) Immunity 6, 107-18. 2 . Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46. 3 . Fujimoto, M. et al. (2000) Immunity 13, 47-57. 4 . ORourke, L.M. et al. (1998) Immunity 8, 635-45. 5 . Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62. |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD19 (D4V4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD19 (D4V4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using CD19 (D4V4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD19 (D4V4B) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells. | |
Western blot analysis of extracts from Daudi cells, untreated (-) or treated with PNGase F (+), using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD19 (D4V4B) XP® Rabbit mAb performed on the Leica® Bond™ Rx. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using CD19 (D4V4B) XP® Rabbit mAb. |
