| 货号 | 67455S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP,IF,F |
| 目标/特异性 | M-CSF Receptor (D3O9X) XP® Rabbit mAb #67455 recognizes endogenous levels of total M-CSF Receptor protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Immunofluorescence (Immunocytochemistry) (1:100) Flow Cytometry (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). |
| 存放说明 | -20C |
| 计算分子量 | 140-200 |
| 参考文献 | 1 . Stanley, E.R. et al. (1978) Nature 274, 168-70. 2 . Byrne, P.V. et al. (1981) J Cell Biol 91, 848-53. 3 . Bourette, R.P. and Rohrschneider, L.R. (2000) Growth Factors 17, 155-66. 4 . Novak, U. et al. (1996) Oncogene 13, 2607-13. 5 . Bourette, R.P. et al. (1997) EMBO J 16, 5880-93. 6 . Morley, G.M. et al. (1999) Oncogene 18, 3076-84. 7 . Toy, E.P. et al. (2001) Gynecol Oncol 80, 194-200. 8 . Maher, M.G. et al. (1998) Clin Cancer Res 4, 1851-6. |
Western blot analysis of extracts from human CD14+ Monocytes, THP-1, and Raji cells using M-CSF Receptor (D3O9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD14+ Monocytes were purified from human blood. | |
Immunoprecipitation of M-CSF Receptor protein from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP®Isotype Control #3900, and lane 3 is M-CSF Receptor (D3O9X) XP® Rabbit mAb. Western blot analysis was performed using M-CSF Receptor (D3O9X) XP®Rabbit mAb. | |
Confocal immunofluorescent analysis of THP-1 (left, positive) and Raji (right, negative) cells using M-CSF Receptor (D3O9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Raji (blue) and THP-1 cells (green) using M-CSF Receptor (D3O9X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragments (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
