| 货号 | 39692S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP,IHC |
| 目标/特异性 | p63 (D9L7L) Rabbit mAb recognizes endogenous levels of total p63 protein. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize all isoforms of p63 except for p63ε. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunohistochemistry (Paraffin) (1:900) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Levine, A.J. (1997) Cell 88, 323-31. 2 . Waltermann, A. et al. (2003) Oncogene 22, 5686-93. 3 . Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7. 4 . Yang, A. et al. (1999) Nature 398, 714-8. |
Western blot analysis of extracts from various cell lines cells using p63 (D9L7L) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of ME-180 cells. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is p63 (D9L7L) XP® Rabbit mAb. Western blot analysis was performed using p63 (D9L7L) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human intraductal and infiltrating carcinoma of the breast using p63 (D9L7L) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded ME-180 cell pellet (left, positive) and MCF7 cell pellet (right, negative) using p63 (D9L7L) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using p63 (D9L7L) XP® Rabbit mAb. |
