| 货号 | 70720S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP,IHC,IF |
| 目标/特异性 | ASS1 (D4O4B) XP® Rabbit mAb recognizes endogenous levels of total ASS1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) Immunohistochemistry (Paraffin) (1:250) Immunofluorescence (Immunocytochemistry) (1:1600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Argininosuccinate synthetase (ASS1) catalyzes the formation of argininosuccinate from citrulline and aspartate, the rate-limiting step in the urea cycle that is responsible for the synthesis of arginine and the clearance of nitrogenous waste (1). ASS1 is ubiquitously and differentially expressed in different cell types and tissues. Mutations in ASS1 are associated with citrullinemia type I, an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels in human patients (2, 3). Loss of ASS1 expression is one of the common metabolic alterations observed in many cancers, and it is a prognostic biomarker of reduced metastasis-free survival. ASS1 deficiency leads to the dependence of extracellular arginine for survival, proliferation, and cell growth. Ariginine starvation induces autophagy and apoptosis in ASS1 deficient cells and this has been exploited as a therapeutic intervention for the tumors with loss of ASS1 expression (4, 5). Pegylated arginine deiminase (ADI-PEG20), an enzyme that degrades arginine into citrulline, causes significant growth inhibition in tumors that have lost ASS1 expression, such as hepatocellular carcinoma, breast cancer, and sarcoma (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 47 |
| 参考文献 | 1 . Haines, R.J. et al. (2011) Int J Biochem Mol Biol 2, 8-23. 2 . Engel, K. et al. (2009) Hum Mutat 30, 300-7. 3 . Woo, H.I. et al. (2014) Clin Chim Acta 431, 1-8. 4 . Delage, B. et al. (2010) Int J Cancer 126, 2762-72. 5 . Feun, L. et al. (2008) Curr Pharm Des 14, 1049-57. 6 . Ensor, C.M. et al. (2002) Cancer Res 62, 5443-50. 7 . Qiu, F. et al. (2014) Sci Signal 7, ra31. 8 . Bean, G.R. et al. (2016) Cell Death Dis 7, e2406. |
Immunohistochemical analysis of paraffin-embedded mouse kidney using ASS1 (D4O4B) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using ASS1 (D4O4B) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ASS1 (D4O4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using ASS1 (D4O4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HSG cell pellet (left, high-expressing) or HT-1080 cell pellet (right, low-expressing) using ASS1 (D4O4B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using ASS1 (D4O4B) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of DLD-1 cells (left, high-expressing) and HT-1080 cells (right, low-expressing) using ASS1 (D4O4B) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
