| 货号 | 8070S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb detects endogenous levels of total GluR 4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:400) |
| 供应商 | CST |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).GluR 4 containing AMPARs are found in synapses and GluR 4 delivery to synapses and cell surface expression is mediated through phosphorylation of Ser842 by PKA or PKC (3). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Confocal immunofluorescent analysis of rat retina using AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠视网膜，使用的抗体是AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb 兔单抗（绿色）。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 | |
Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb. Western blot分析小鼠大脑、NIH/3T3细胞、大鼠大脑和C6细胞提取物，使用的抗体是AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb 兔单抗。 |
