| 货号 | 5053S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Argonaute 1 (D84G10) XP® Rabbit mAb detects endogenous levels of total Argonaute 1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1000) |
| 供应商 | CST |
| 背景 | Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing such as repression of translation and mRNA turnover (1). |
| 存放说明 | -20C |
| 计算分子量 | 97 |
Immunohistochemical analysis of paraffin-embedded human breast using Argonaute 1 (D84G10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用Argonaute 1 (D84G10) XP®Rabbit mAb，免疫组化分析人类乳腺组织石蜡切片，切片分别孵育对照多肽 (左图)和抗原特异性多肽(右图)。 | |
Western blot analysis of extracts from various cell types using Argonaute 1 (D84G10) XP® Rabbit mAb. 使用Argonaute 1 (D84G10) XP® Rabbit mAb兔单抗，免疫印迹（Western blot）分析不同细胞系提取物。 |
