| 货号 | 5019S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | ASH2L (D93F6) XP® Rabbit mAb detects endogenous levels of all known isoforms of the ASH2L protein. |
| 使用方法 | WB(1:2000) IP (1:100) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6). |
| 存放说明 | -20C |
| 计算分子量 | 80, 65 |
Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb. 使用ASH2L (D93F6) XP® Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中ASH2L蛋白水平。 | |
Confocal imunofluorescent analysis of HeLa cells using ASH2L (D93F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用ASH2L (D93F6) XP® Rabbit mAb 兔单抗(绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
