| 货号 | 8178S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | C/EBPα (D56F10) XP® Rabbit mAb recognizes endogenous levels of total C/EBPα protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4). |
| 存放说明 | -20C |
| 计算分子量 | 42, 28 |
Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using C/EBPα (D56F10) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对分化的3T3-L1细胞使用C/EBPα (D56F10) XP?兔单克隆抗体（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Confocal immunofluorescent analysis of THP-1 (left) and Jurkat (right) cells using C/EBPα (D56F10) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对THP-1细胞（左）和Jurkat细胞（右）使用C/EBPα (D56F10) XP?兔单克隆抗体（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil using C/EBPα (D56F10) XP® Rabbit mAb. 对石蜡包埋的人扁桃体使用C/EBPα (D56F10) XP?兔单克隆抗体进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse lung using C/EBPα (D56F10) XP® Rabbit mAb. 对石蜡包埋的鼠肺使用C/EBPα (D56F10) XP?兔单克隆抗体进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, THP-1 (left) or Jurkat (right), using C/EBPα (D56F10) XP® Rabbit mAb. 对石蜡包埋细胞团，THP-1(左)或Jurkat(右)，使用C/EBPα (D56F10) XP? Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using C/EBPα (D56F10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人肝细胞肝癌使用C/EBPα (D56F10) XP?兔单克隆抗体进行免疫组织化学分析，其中左图对照肽，右图抗原特异肽。 | |
Western blot analysis of extracts from Hep G2 and LNCaP cells using C/EBPα (D56F10) XP® Rabbit mAb. 对Hep G2和LNCaP细胞抽提液使用C/EBPα (D56F10) XP?兔单克隆抗体进行Western blot分析。 |
