| 货号 | 6502S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | C1QBP (D7H12) XP® Rabbit mAb recognizes endogenous levels of total C1QBP protein. |
| 使用方法 | WB(1:1000) IHC-P (1:1600) F (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | C1QBP, also referred to as p32, p33, gC1q receptor (gC1qR), and hyaluronic acid binding protein 1 (HABP1), was originally identified via its binding interactions with Splicing Factor (SF-2) (1). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases (2), the tumor suppressor ARF (3-5), and multiple antigens of bacterial and viral origin (6). Research studies have shown that C1QBP is overexpressed in a number of cancer cell types (7), and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis (7). C1QBP has also been shown to inhibit the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress (8). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Western blot analysis of extracts from various cell lines using C1QBP (D7H12) XP® Rabbit mAb. Wetern blot检测多种细胞系提取物，所用抗体为C1QBP (D7H12) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using C1QBP (D7H12) XP® Rabbit mAb. 免疫组织化学分析石蜡包埋的人乳腺癌细胞，使用抗体为C1QBP (D7H12) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse ovary using C1QBP (D7H12) XP® Rabbit mAb. 免疫组织化学分析石蜡包埋的小鼠卵巢，使用抗体为C1QBP (D7H12) XP® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of HeLa cells using C1QBP (D7H12) XP® Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa 细胞，使用抗体为 C1QBP (D7H12) XP® Rabbit mAb 兔单抗(绿色) 和β-Catenin (L54E2) Mouse mAb 鼠单抗(Alexa Fluor® 555 Conjugate) #5612 (红色)。 蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Flow cytometric analysis of Hela cells using C1QBP (D7H12) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. 流式细胞仪分析Hela细胞，使用C1QBP (D7H12) XP® Rabbit mAb兔单抗（蓝色）对照浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红色）。使用二抗为Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412。 |
