货号 | 12980S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP/IF-IC |
使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
供应商 | CST |
背景 | Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen (1). Research studies show that overexpression of this antioxidant enzyme increases the ability of pancreatic β-cells to scavenge reactive oxygen species (ROS), thereby protecting pancreatic β-cells from oxidative stress (2). The pancreatic β-cells overexpressing this enzyme are also protected from hydrogen peroxide-mediated lipotoxicity, providing further evidence for the importance of catalase in the pathogenesis of diabetes (3). |
存放说明 | -20C |
计算分子量 | 60 |
Confocal immunofluorescent analysis of HeLa cells using Catalase (D4P7B) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Catalase (D4P7B) XP® Rabbit mAb兔单抗 (绿色)对HeLa细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054 (红色)标记。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from Hep G2, HeLa, and 293 cells using Catalase (D4P7B) XP® Rabbit mAb. 使用Catalase (D4P7B) XP® Rabbit mAb兔单抗对Hep G2, HeLa, 和293细胞提取物进行western blot分析。 | |
Immunoprecipitation of catalase from extracts of HeLa cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Catalase (D4P7B) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Catalase (D4P7B) XP® Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 Catalase (D4P7B) XP® Rabbit mAb (lane 3)对HeLa细胞提取物中的过氧化氢酶进行免疫沉淀分析。Lane1是10%上样量。使用Catalase (D4P7B) XP® Rabbit mAb兔单抗进行western blot分析。 |