| 货号 | 3267S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Bovine/Dog |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | Caveolin-1 (D46G3) XP® Rabbit mAb detects endogenous levels of total caveolin-1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:800) F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). |
| 存放说明 | -20C |
| 计算分子量 | 21, 24 |
Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb. 对多种细胞系抽提液使用Caveolin-1 (D46G3) XP® Rabbit mAb进行Western blot分析。 | |
Immunoprecipitation of caveolin-1 from HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input. 使用Caveolin-1 (D46G3) XP® Rabbit mAb对HeLa细胞提取物进行caveolin-1的免疫共沉淀，然后在进行western检测。泳道1是5%的input。 | |
Immunohistochemical analysis of paraffin-embedded mouse lung using Caveolin-1 (D46G3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的小鼠肺使用Caveolin-1 (D46G3) XP® Rabbit mAb兔单抗进行免疫组织化学分析，其中左图加入对照肽，右图加入抗原特异肽。 | |
Flow cytometric analysis of Hela cells using Caveolin-1 (D46G3) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 使用Caveolin-1 (D46G3) XP® Rabbit mAb兔单抗（蓝）和非特异的阴性对照抗体（红），对Hela细胞进行流式细胞术分析。 | |
Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 (D46G3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). 对C2C12细胞使用Caveolin-1 (D46G3) XP® Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Caveolin-1 (D46G3) XP® Rabbit mAb. 对石蜡包埋的人结肠癌使用Caveolin-1 (D46G3) XP® Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Caveolin-1 (D46G3) XP® Rabbit mAb. 对石蜡包埋的人淋巴瘤使用Caveolin-1 (D46G3) XP® Rabbit mAb兔单抗进行免疫组化分析。 |
