| 货号 | 8522S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Caveolin-2 (D4A6) XP® Rabbit mAb recognizes endogenous levels of total caveolin-2 protein. Based on amino acid sequence, the antibody is not expected to cross-react with caveolin-1 or caveolin-3. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Confocal immunofluorescent analysis of HeLa cells using Caveolin-2 (D4A6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Caveolin-2 (D4A6) XP® Rabbit mAb（绿色）对HeLa细胞进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Caveolin-2 (D4A6) XP® Rabbit mAb.使用Caveolin-2 (D4A6) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of caveolin-2 from HeLa cell extracts using Caveolin-2 (D4A6) XP® Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 10% input.使用Caveolin-2 (D4A6) XP® Rabbit mAb对HeLa细胞提取物中的小窝蛋白-2进行免疫沉淀实验。使用同样的抗体进行western blot分析。Lane1是10%的上样量。 |
