| 货号 | 85061S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) |
| 供应商 | CST |
| 背景 | When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Immunohistochemical analysis of paraffin-embedded human tonsil using CD3ε (D7A6E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded MOLT-4 (left) and PC-3 (right) cell pellets using CD3ε (D7A6E) XP® Rabbit mAb. | |
Western blot analysis of extracts from MOLT-4, HuT 102, and PC-3 cells using CD3ε (D7A6E) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD3ε (D7A6E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins lymphoma using CD3ε (D7A6E) XP® Rabbit mAb. |
