| 货号 | 4793S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | CDC37 (D11A3) XP® Rabbit mAb detects endogenous levels of total CDC37 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | CDC37 is an important component of the HSP90 chaperone complex (1,2). It was initially identified for its involvement in cell-cycle progression and was later found to have a much broader role as a chaperone for a wide variety of kinases and other proteins (1-3). CDC37 protein has an amino-terminal kinase binding domain followed by a central HSP90 binding domain. It recruits and stabilizes kinases in the HSP90 complex by protecting the newly synthesized kinase peptide chain from degradation and promoting the next step of protein maturation (4,5). CDC37 also suppresses the ATPase activity of HSP90, thereby leading to conformational changes in the complex that preclude target kinase loading (6). CDC37 has been proposed as a therapeutic target because of its important role in multiple kinase pathways involved in proliferation and cancer cell survival, including Raf, Akt, Src, and ErbB2 pathways (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or EGF-treated (#8916, 100 ng/mL for 5 minutes; right), using CDC37 (D11A3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞，血清饥饿处理（左）或EGF处理（#8916，100ng/ml，5分钟）（右），使用CDC37 (D11A3) XP® Rabbit mAb（绿色）兔单抗进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using CDC37 (D11A3) XP® Rabbit mAb. 对多种细胞系提取物使用CDC37 (D11A3) XP® Rabbit mAb兔单抗进行Western blot分析。 |
