| 货号 | 5480S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | CHAF1A (D77D5) XP® Rabbit mAb detects endogenous levels of total CHAF1A protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Confocal immunofluorescent analysis of HeLa cells using CHAF1A (D77D5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用CHAF1A (D77D5) XP® Rabbit mAb兔单抗 (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cell lines using CHAF1A (D77D5) XP® Rabbit mAb. 使用CHAF1A (D77D5) XP® Rabbit mAb兔单抗，免疫印迹(Western blot)分析HeLa、Jurkat和COS-7细胞中CHAF1A蛋白水平。 |
