| 货号 | 62257S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:300) |
| 供应商 | CST |
| 背景 | Doublecortin-like kinase 1 (DCLK1, DCAMKL1) is a serine/threonine kinase that belongs to the CaM kinase family and shares homology with the neuronal microtubule binding protein doublecortin. DCLK1 is thought to be involved in calcium signaling pathways controlling neuronal development in the embryonic brain (1,2). The kinase also functions in the mature nervous system and is highly expressed in regions of active neurogenesis in the neocortex and cerebellum (3,4). Research studies suggest that the DCLK1 kinase is highly expressed in subpopulations of cells within the colon and gastric epithelium and in the pancreas (5-8). The nature of these cell populations, whether normal, stem-like, or tumor-initiating, is unclear. |
| 存放说明 | -20C |
| 计算分子量 | 82 |
Immunohistochemical analysis of paraffin-embedded TT (left) and HeLa (right) cell pellets using DCLK1/DCAMKL1 (D2U3L) XP®Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using DCLK1/DCAMKL1 (D2U3L) XP®Rabbit mAb. | |
Western blot analysis of extracts from various cell lines and tissues using DCLK1/DCAMKL1 (D2U3L) XP®Rabbit | |
mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells have extremely low expression of DCLK1/DCAMKL1. | |
Immunoprecipitation of DCLK1/ DCAMKL1 from TT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is DCLK1/DCAMKL1 (D2U3L) XP®Rabbit mAb. Western blot analysis was performed using | |
DCLK1/DCAMKL1 (D2U3L) XP®Rabbit mAb. |
