| 货号 | 11954S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | DLST (D22B1) XP® Rabbit mAb recognizes endogenous levels of total DLST protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Reduction of DLST increases reactive oxygen species production, suggesting its role in oxidative stress (2). Research has shown that deficiency of DLST in mice is linked to increased oxidative stress in mitochondria, a process that may be involved in the pathogenesis of Alzheimers disease (2). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Confocal immunofluorescent analysis of HeLa cells using DLST (D22B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，使用DLST (D22B1) XP® Rabbit mAb兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白微丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using DLST (D22B1) XP® Rabbit mAb. Wetern blot检测多种细胞系提取物，所用抗体为DLST (D22B1) XP® Rabbit mAb兔单抗。 | |
Immunoprecipitation of DLST from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DLST (D22B1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DLST (D22B1) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies. 免疫沉淀方法检测293细胞提取物DLST蛋白，使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或DLST (D22B1) XP® Rabbit mAb 兔单抗(泳道3)。泳道1为10%对照。使用DLST (D22B1) XP® Rabbit mAb 兔单抗进行Western blot方法分析，二抗是Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 和Anti-mouse IgG, HRP-linked Antibody #7076。 |
